孕激素对输卵管上皮细胞早期癌化的抑制作用及机制探讨

来源 :中国工程院2014年医学科学前沿论坛第十四次全国临床药理学学术会议暨第六届国际药物警戒与药物安全学术会议 | 被引量 : 0次 | 上传用户：qncy1235p

【摘要】

：

目的:本研究的主要目的是探讨孕激素对输卵管早期癌化细胞的抑制作用,及其抑癌机制的探讨.方法:用接近血浆浓度和接近滤泡液浓度的孕激素处理输卵管上皮原代细胞和输卵管上皮早期癌化细胞FE25(TP53突变细胞),(1)通过MTT检测不同细胞存活率;(2)通过显微镜拍照观察细胞的死亡形态;(3)通过流式细胞仪检测细胞的凋亡状况;(4)通过流式细胞仪检测细胞周期的抑制情况;(5)通过检测LDH检测细胞的坏死

【作者】

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吴娜怡园黄玄舜许泽芳秦崇臻朱堂元周宏灏

【机构】

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中南大学湘雅医院临床药理研究所,长沙410008 中南大学临床药理研究所,遗传药理学湖南省重点实验

【出处】

：

中国工程院2014年医学科学前沿论坛第十四次全国临床药理学学术会议暨第六届国际药物警戒与药物安全学术会议

【发表日期】

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2014年3期

【关键词】

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孕激素 FE25 输卵管早期癌化 MMP9 NF-kB

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孕激素对输卵管上皮细胞早期癌化的抑制作用及机制探讨

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