肝细胞生长因子对低密度脂蛋白损伤内皮祖细胞功能的影响

来源 :第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int | 被引量 : 0次 | 上传用户：acxyvpfag

【摘要】

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目的:探讨肝细胞生长因子对低密度脂蛋白损伤内皮祖细胞功能过程中的作用.方法:采用梯度离心法分离并培养大鼠骨髓EPCs,DiI-acLDL摄取和FITC-UEA-I结合荧光双染法鉴定大鼠EPCs.培养的内皮祖细胞给予不同浓度(20,40,and 60μg/mL)的LDL胆固醇刺激.MTT、改良boyden小室及annexin V流式细胞检测技术来评价内皮祖细胞的增殖、迁移及凋亡.通过观察AKT及其磷

【作者】

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赵刚黄岚

【机构】

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第三军医大学新桥医院全军心血管病研究所

【出处】

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第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int

【发表日期】

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2013年2期

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肝细胞生长因子对低密度脂蛋白损伤内皮祖细胞功能的影响

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