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Today, intracytoplasmic sperm injection (ICSI) has been routinely used as a most effective treatment procedure for severe male factor infertility.In routine ICSI, a single motile sperm is subjectively selected by a scientist and it still can be difficult to select a most competent sperm from general motile population, particularly those patients with severe sperm defects and high sperm DNA damage.Thus about 5-10％ ICSI patients still have persistently poor outcomes.