【摘 要】
：
[Objective]Tetrodotoxin(TTX) is a highly toxic low-molecular and non-protein marine biotoxin,and it is also one of the most toxic neurotoxin in nature.Solid-phase microextraction(SPME) is a new extrac
【机 构】
：
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306,China
论文部分内容阅读
[Objective]Tetrodotoxin(TTX) is a highly toxic low-molecular and non-protein marine biotoxin,and it is also one of the most toxic neurotoxin in nature.Solid-phase microextraction(SPME) is a new extraction technique which is based on the solid-phase extraction(SPE).It is a system which includes sampling,extraction,concentration,with the advantage of simple operation,rapid,low cost,safety,good choice,high sensitive and solventlessness.To verify the possibility that tetrodotoxin could be extracted from its standard using solid-phase microextraction and determine its extraction efficiency,the experiment was conducted.[Methods]First,the tetrodotoxin standard was diluted by mobile phase which was made of water,acetic acid and tetrahydrofuran(v∶v∶v=1000∶1.5∶5),forming solutions with three different concentrations(40mg/L、 20mg/L and 10mg/L).Then solid-phase microextraction and elution were conducted in bottles under ambient temperature,which lasted 30min and 20min respectively.The SPME fiber used was 85μm polyacrylate(PA).Finally,the absorbance values of TTX at 196.1 nm were obtained from UV spectrophotometer.[Results]Calibration curve was linear over the TTX concentration range of 15~40mg/L(y=0.005x+ 1.589,r=0.9654).[Conclusion]It was concluded that TTX could be extracted from its standard using solid-phase microextraction,but the extraction efficiency was not satisfactory enough due to the short extraction and elution time.
其他文献
This thesis proposes two supply system on plate freezer that one is a type of ejector throttling refrigeration system (Mode Ⅰ) and another is the type of thermal expansion valve (Mode Ⅱ),studies the e
[Objective]To find out the appropriate apple cultivars for processing pure micropowder,we evaluated the physicochemical and flowability qualities of different pure apple micropowders which were proces
[Objective]To determine the microorganisms and their percentage respectively in the Antarctic krill,a simple flora analysis of the cultured microorganisms in the Antarctic krill were examined.[Methods
Pacific white shrimp (Litopenaeus vannamei) is widely cultured and commercially viable in many parts of the globe particularly in China,Malaysia,Thailand,etc.,probably because of its positive quality
[Objective]Raman scattering and microscopic observations (Confocal laser scanning microscopy (CLSM) and Scanning Electron Microscopy(SEM)) have recently been proved to be a promising technique for cha
Low molecular mass (MM)chitosan with high degree of deacetylation (DDA) has excellent bioactivity including targeting drug delivery,antibacterial and encapsulation properties.Thus,to reduce MM of chit
The maturity discrimination of hepatopancreas and gonadal development of Eriocheir sinensis always is a big challenge,and cannot be solved by conventional method,including needle puncture and naked ey
Nowadays foodbome diseases mainly caused by food borne pathogens are emerging public health concern worldwide.Thus,fast and effective approaches for detection the pathogens are vital.It is known that
Pacific saury (Cololabis saira) is one of the most important marine commercial fishes in the world.The viscera are rich in lecithin.In this study,the Pacific saury viscera was extracted by supercritic
[Objective]Quality changes of frozen mango cuboids in long-term glassy state storage with and without osmotic dehydration pretreatment were investigated.[Methods]Mango cuboids were dehydrated in mixed