海南三七离体快繁的研究

来源 :2013年广东省研究生学术论坛——园艺分论坛 | 被引量 : 0次 | 上传用户:a18102023
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以海南三七(Kaempferia rotunda)的芽、假茎基部、叶鞘为材料,通过对细胞分裂素6-BA、kinetin和生长素NAA、2,4-D对其外植体作用的研究,建立直接和间接植株再生体系.结果表明:快繁最佳外植体为海南三七的芽和假茎基部;芽增殖最佳培养基为MS+6-BA2.0mg/L+NAA 0.10mg/L,增殖培养最佳激素比例:6-BA与NAA浓度比值为20倍:茎基部外植体接种在MS +6-BA2.0 mg/L+2,4-D1.0 mg/L的愈伤诱导培养基上愈伤诱导率最高,50天后达78.43%:将诱导出的愈伤接种在M.S+6-BA 6.0 mg/L+NAA 0.7 mg/L的分化培养基上,分化率可以达到66.67%;将组培苗移栽至基质中,成活率100%.
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