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Microfluidic chip has been performed as a platform of various chemical reactions with high efficiency due to the large surface to volume ratio and shorter diffusion distance of reactant solution in microchannels.In this work, we presented a novel microfluidic-based photocatalytic microreactor by using electrospun nanofibrous TiO2 as the photocatalyst.The micoreactor is simply fabricated by sealing the nanofibrous TiO2 between the polydimethylsiloxane (PDMS) substrate and glass slide.