Collagen is a kind of natural polymer material,which has the advantages of biocompatibility,non-antigenicity,non-toxicity,good hemostatic effect and biodegradation.However,its mechanical properties are not high enough,the biodegradation speed is fast and is easily denaturation.Therefore its mechanical properties need to further improve.In this work,self-assembly of extracted collagen from swine trotter tendon under different conditions was firstly observed using atomic force microscopy;then the effects of glutaraldehyde concentrations,UV,and UV with riboflavin to the topography of the collagen assembly were analyzed with the height images and section analysis data.Collagen were treated with 0.02 %,0.04 %,0.10 %,0.20%glutaraldehyde,the width of collagen was first increased and the decreased along with the increased of glutaraldehyde concentration,on the contrary,the change of height showed an obviously trend.0.1 and 1%riboflavin photo-activated treat with collagen for 1 h,the flluorescent as a control group,the width,height and roughness have an significant difference.These results and analysis would be helpful for collagen assembly on plastic wrap with a potential application in food science and engineering.