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小鹅瘟是雏鹅感染鹅细小病毒(Goose plaguevirus,GPV)所引起的一种急性败血型传染病,病程短且死亡率很高,主要侵害1月龄以内的雏鹅和雏番鸭。目前,市场销售的对此病进行免疫和治疗的抗小鹅瘟血清存在产量低、成本高,且容易导致某些鹅源疫病的传播的缺点。笔者采用从贵州铜仁市等地的养鹅场采集的病鹅分离出的小鹅瘟野生强毒株接种于9日龄的鹅胚尿囊液制备了小鹅瘟病毒抗原,并用小鹅瘟病毒抗原加弗氏佐剂后,分多次皮下注射结合静脉注射小鹅瘟病毒抗原免疫贵州黑、白山羊。颈动脉一次性放血制备抗小鹅瘟异源高免血清,用琼脂扩散进行效价的测定,结果显示其抗体效价平均都在1∶8以上。将制备的血清与市售的抗小鹅瘟进行了对比试验,结果市售的抗小鹅瘟血清的保护率为85%,山羊抗小鹅瘟高免血清的保护率为95%。山羊抗小鹅瘟异源高免血清具有效价高、成本低、安全性好等优点。
Gosling is a kind of acute sepsis caused by gosling in goose plague virus (GPV). Its short duration and high mortality rate mainly affect goslings and young muscovy ducks up to 1 month old. Currently, anti-gosling serums marketed to immunize and treat this disease have the disadvantage of low yield, high cost, and easily leading to the spread of some geese disease. I used from Tongren City, Guizhou Province and other places collected goose geese goose gosling isolated wild goose virulent strains were inoculated 9-day-old goose embryo allantoic fluid prepared gosling pestivirus antigen and gosling plague virus Antigen plus Freund’s adjuvant, multiple subcutaneous injection of intravenous injection of gosling plague virus antigen Guizhou black and white goats. One-time carotid blood was prepared for anti-gosling hematoxylin-eosin high serum, agar diffusion titer was measured, the results showed that the average antibody titer in more than 1: 8. The prepared sera were compared with the commercially available anti gosling plague. The results showed that the protective rate of the commercially available anti-gosling plague serum was 85%, and that of goat anti-gosling plague serum was 95%. Goat anti gosling plague heterologous high serum with high potency, low cost, good safety and so on.