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以NaBH4为还原剂,制备了金与铂物质的量比为49∶1的金铂纳米合金(GP).用兔抗人绒毛膜促性腺激素抗体(RhCG)修饰AuPt获得了免疫纳米合金探针(GP-RhCG).在pH 5.8磷酸氢二钠-柠檬酸缓冲溶液及KCl存在的条件下,GP-RhCG探针发生非特异性聚集,在590 nm处有一个较强的共振散射峰.当有人绒毛膜促性腺激素(hCG)存在时,聚集的GP-RhCG探针与hCG发生特异性结合,生成分散性较好的GP-RhCG-hCG免疫复合物,导致590 nm处共振散射峰强度降低.其共振散射峰强度降低值ΔI590 nm与hCG浓度在6.67～86.7 ng/mL范围内呈现良好线性关系.免疫反应液中形成的GP-RhCG-hCG免疫复合物对葡萄糖-铜(II)体系具有较强的催化作用,其产物在610 nm处有一较强共振散射峰.随着hCG浓度增大,形成的GP-RhCG-hCG复合物越多,其催化作用增强,610 nm处的共振散射峰增强.其共振散射峰增大值ΔI610 nm与hCG浓度在3.33～133 ng/mL范围内呈线性关系.
NaBH4 was used as reductant to prepare gold-platinum nano-alloy (GP) with the ratio of gold to platinum of 49:1. Immuno-nanometer alloy probe was obtained by modifying AuPt with rabbit anti-human chorionic gonadotropin antibody (RhCG) (GP-RhCG). GP-RhCG probe was nonspecifically aggregated at pH 5.8 in the presence of disodium hydrogen phosphate-citrate buffer and KCl, and had a strong resonance scattering peak at 590 nm. In the presence of human chorionic gonadotropin (hCG), the aggregated GP-RhCG probe specifically bound to hCG to produce a well-dispersed GP-RhCG-hCG immune complex, resulting in a decrease in the intensity of the resonant scattering peak at 590 nm. The linear decrease of resonance scattering intensity ΔI590 nm and hCG concentration showed a good linear relationship in the range of 6.67-86.7 ng / mL.The GP-RhCG-hCG immune complexes formed in the immunoreaction system had a better effect on the glucose-copper (II) system Strong catalysis and a strong resonance scattering peak at 610 nm.With the increase of hCG concentration, the more GP-RhCG-hCG complex formed, the stronger catalytic activity and the enhanced resonance scattering peak at 610 nm . The resonance scattering peak increase ΔI610 nm and hCG concentration in the range of 3.33 ~ 133 ng / mL linear relationship.