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应用差异ＰＣＲ技术研究了大肠癌中Ｃ－ｅｒｂＢ－２基因的扩增情况。结果发现，大肠癌组织中Ｃ－ｅｒｂＢ－２基因存在明显扩增，该基因的扩增与大肠癌组织学分级、远处转移之间均有非常显著的关联。大肠癌中随着癌组织学分级程度的加重，Ｃ－ｅｒｂＢ－２基因扩增逐渐向高、低拷贝转移，说明，Ｃ－ｅｒｂＢ－２基因扩增在一定程度上反映着大肠癌分化状态和生物学行为，可作为大肠癌患者预后的重要参数，并可指导临床治疗。
Differential PCR was used to study the amplification of C-erbB-2 gene in colorectal cancer. The results showed that there was a significant amplification of C-erbB-2 gene in colorectal cancer tissues, and there was a very significant correlation between the amplification of this gene and the histological grading and distant metastasis of colorectal cancer. With the aggravation of histological grade of colorectal cancer, C-erbB-2 gene amplification gradually shifted to high and low copy, indicating that C-erbB-2 gene amplification reflects the differentiation status of colorectal cancer to some extent. Biological behavior can be an important parameter for the prognosis of patients with colorectal cancer and can guide clinical treatment.