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目的:以刺梨果总多酚为考察指标,对贵州省不同来源和不同干燥方式刺梨果的品质进行研究,为其规范化种植、干燥、储存提供科学数据。方法:采用UV测定总多酚含量,以没食子酸为指标成分,通过单因素试验优选显色条件。测定26批不同来源样品中总多酚的含量,考察3种干燥方式对总多酚含量的影响,通过聚类分析法对测定结果进行系统处理和分析。结果:没食子酸在2.5~5.4 mg·L-1线性关系良好(r=0.999 7),平均加样回收率102.93%(RSD 3.3%)。26批样品中总多酚质量分数平均值10.06%,其中实验室采集自制样品平均含量为10.77%(n=22),4批市售样品均值6.14%(n=4)。60℃烘干、冷冻干燥和40℃减压干燥的样品中总多酚质量分数分别下降了33.57%,23.96%和24.38%。结论:建立的刺梨果中总多酚的含量测定方法简便、重复性好,可为提升地方标准提供参考。不同干燥方式对刺梨果中总多酚含量的影响存在一定差异,冷冻干燥和40℃减压干燥效果相对较佳。
OBJECTIVE: To study the quality of prickly pear fruit from different origins and different ways of drying in Guizhou Province, and to provide scientific data for its standardized planting, drying and storage. Methods: The total polyphenols content was determined by UV. Gallic acid was used as the index component. The color condition was optimized by single factor test. The content of total polyphenols in 26 batches of different sources samples was determined. The effects of three drying methods on the total polyphenols contents were investigated. The results were systematically analyzed and analyzed by cluster analysis. Results: The linearity of gallic acid was 2.5 ~ 5.4 mg · L-1 (r = 0.999 7). The average recovery was 102.93% (RSD 3.3%). The average content of total polyphenols in 26 batches of samples was 10.06%. The average content of homemade samples in laboratory was 10.77% (n = 22) and the average of four batches of samples was 6.14% (n = 4). The total polyphenols content in the samples dried at 60 ℃, freeze-dried and dried under reduced pressure at 40 ℃ decreased by 33.57%, 23.96% and 24.38%, respectively. Conclusion: The method for determination of total polyphenols in Rosa roxburghii is simple, reproducible and can provide a reference for improving local standards. The effect of different drying methods on the total polyphenol content of the Rizuki pear fruit is different, freeze-drying and 40 ℃ decompression drying effect is relatively good.