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转基因动物的建立是一项复杂而艰苦的工作，在转基因胚移植受体前对其进行检测，无疑对转基因动物建立具有重要意义。使用小鼠乳清酸蛋白（WAP）控制下的人粒细胞激落刺激因子（G-CSF）基因为显微注射片段，采用PCR方法检测了转基因胚，在1、2和8细胞期的阳性率为100％、77．7％和44．4％。为消除PCR扩增中的假阳性结果，构建了两个具有部分同源性的亚克隆片段进行共注射。PCR扩增片段跨越这一同源区域，转基因的非整合胚不能扩增出特异性片段。结果表明，1、2和8细胞期的阳性率分别为11．1％、55．5％和44．4％，较常规PCR检测获得更为明确的结论，为在大动物转基因的检测提供了新依据。
The establishment of transgenic animals is a complicated and arduous task. Detection of the transgenic embryos before they are transplanted to recipients will undoubtedly have great significance for the establishment of transgenic animals. The human granulocyte-stimulating factor (G-CSF) gene under the control of mouse whey protein (WAP) was used as a microinjection fragment. The transgenic embryos were detected by PCR and were positive at 1, 2 and 8 cell stage Rates were 100%, 77.7% and 44.4%. To eliminate the false-positive result in PCR amplification, two subclone fragments with partial homology were constructed for co-injection. PCR amplified fragment across this homologous region, non-genetically modified embryos can not be amplified specific fragments. The results showed that the positive rates of 1, 2 and 8 were 11.1%, 55.5% and 44.4%, respectively, which were more clear than conventional PCR. New basis.