论文部分内容阅读
以pBI121质粒作外源DNA,去壳水稻种子为浸泡受体。对G418抗性苗进行Southernblot分析,证实pBI121上的NPT正基因整合到受体基因组中。以溶解PBI121的TE溶液浸泡处理为对照,用扫描电镜观察胚芽生长点外层细胞表面,结果显示,对照与DNA溶液浸泡组两者间细胞表面结构有相似性,但DNA溶液浸泡组出现对照组所没有的表面孔洞结构,说明DNA可诱导生长点细胞表面产生新的结构。
The pBI121 plasmid was used as exogenous DNA, and the dehulling rice seeds were immersed in the receptor. Southern blot analysis of G418 resistant seedlings confirmed that the NPT positive gene on pBI121 was integrated into the recipient genome. The solution of PBI121 dissolved in TE solution was used as a control, and the surface of the outer layer of embryo growth spots was observed by scanning electron microscopy. The results showed that there were similar cell surface structures between control and DNA solution immersion groups, but DNA solution immersion group appeared control group The lack of surface pore structure, indicating that DNA can induce the growth of cell surface to generate new structures.