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本研究用PAP法、胸腺细胞增殖法、脾细胞增殖法,分别检测16例体外HBV感染的骨髓单个核细胞与16例慢性乙型肝炎患者体内感染的骨髓单个核细胞(MNCs)中的HBcAg和白细胞介素-1(IL-1)、白细胞介素-2(IL-2)的诱生活性(以△cpm值表示)。结果显示,体外HBV感染组与体内HBV感染组骨髓MNCs中HBcAg检出率分别为50％和43.7％。本实验结果表明,HBV在体外感染骨髓MNCs,且与体内自然感染相符,但光镜下未观察到致细胞病变效应(CPE)。体外感染组与体内感染组IL-I和IL-2活性均较对照组明显下降(P<0.01)。且细胞中HBcAg检出阳性者较阴性者下降更为明显(P<0.01)。IL-1和IL-2诱生活性降低与HBV侵染免疫细胞及其在细胞内复制有密切关系,从而提示,IL-1和IL-2降低可能影响HBV的清除而引起慢性化过程。
In this study, PAP, thymocyte proliferation and spleen cell proliferation were used to detect the expression of HBcAg and Bcl-2 in bone marrow mononuclear cells (MNCs) from 16 HBV infected bone marrow mononuclear cells and 16 chronic hepatitis B patients, respectively Intrinsic activity of IL-1 and IL-2 (expressed as △ cpm values). The results showed that the detection rate of HBcAg in bone marrow MNCs in vitro and in vivo HBV infection were 50% and 43.7% respectively. The experimental results show that HBV in vitro infection of bone marrow MNCs, and consistent with the natural infection in vivo, but under the light microscope, no cytopathic effect (CPE) was observed. The activity of IL-1 and IL-2 in in vitro infection group and in vivo infection group were significantly lower than those in control group (P <0.01). And HBcAg positive cells were more significantly decreased than the negative (P <0.01). The reduced activity of IL-1 and IL-2 inducing is closely related to HBV infection of immune cells and their intracellular replication, suggesting that the decrease of IL-1 and IL-2 may affect the clearance of HBV and cause the chronic process.