论文部分内容阅读
背景 新血管形成的概念最初用于解释一些免疫性疾病如糖尿病、视网膜病或类风湿性关节炎的发病机制,现已扩展到其他研究领域,如心肌缺血和肿瘤发生。另外,有报导认为内皮祖细胞(EPC)有使血管移植物异处生长的能力。在本研究中,作者从脐血CD34+细胞中分离EPC,并在体外不同的细胞因子条件下培养,以生成内皮细胞,作为治疗性新血管形成的来源。方法 脐带血从18名正常足月分娩的脐带采集到含有CPDA—1的
Background The concept of neovascularization was originally used to explain the pathogenesis of some immunological diseases such as diabetes, retinopathy or rheumatoid arthritis and has now been extended to other areas of research such as myocardial ischemia and tumorigenesis. In addition, it has been reported that endothelial progenitor cells (EPCs) have the ability to heterotrophic vascular grafts. In the present study, the authors isolated EPCs from cord blood CD34 + cells and cultured them under different cytokine conditions in vitro to generate endothelial cells as a source of therapeutic neovascularization. Methods Cord blood was collected from 18 normal full term umbilical cords to CPDA-1 containing