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目的探讨K-ras蛋白与p33ING1蛋白、CyclinE在直肠癌组织中表达情况及其与直肠癌生物学行为的关系。方法运用S-P免疫组化技术,检测72例直肠癌组织及正常直肠黏膜组织中K-ras蛋白与p33ING1蛋白、CyclinE的表达。结果 K-ras与p33ING1、CyclinE的蛋白表达阳性率在直肠癌组织中分别为62.5%、29.2%、72.2%,在正常直肠黏膜中分别为2.8%、91.7%、18.1%,差异有统计学意义(P<0.05);K-ras蛋白在直肠癌组织中的表达与年龄、肿瘤大小、临床分型、组织分化无关;与淋巴结转移及Dukes分期有关,不同分组间的K-ras蛋白表达率差异有统计学意义(P均<0.05);K-ras蛋白表达和p33ING1蛋白表达呈负相关(rs=-0.404,P<0.05),K-ras蛋白表达和CyclinE表达呈正相关(rs=0.571,P<0.05)。结论直肠癌组织中K-ras蛋白、CyclinE表达增高,抑癌基因蛋白p33ING1表达降低,提示K-ras与p33ING1、CyclinE参与直肠癌的发生、发展,并在直肠癌形成过程中起着重要作用。并可能为直肠癌的早期诊断和治疗提供新的方法。
Objective To investigate the expression of K-ras protein, p33ING1 protein and CyclinE in rectal cancer and its relationship with the biological behavior of rectal cancer. Methods The expressions of K-ras protein, p33ING1 protein and CyclinE in 72 cases of rectal cancer and normal rectal mucosa were detected by S-P immunohistochemistry. Results The positive rates of K-ras, p33ING1 and CyclinE in rectal cancer tissues were 62.5%, 29.2% and 72.2% respectively, which were 2.8%, 91.7% and 18.1% respectively in rectal mucosa, the difference was statistically significant (P <0.05). The expression of K-ras protein in rectal cancer tissues was not related to age, tumor size, clinical classification and histological differentiation, but also to the lymph node metastasis and Dukes staging. The difference of K-ras protein expression rates among different groups (Rs = -0.404, P <0.05). There was a positive correlation between K-ras expression and CyclinE expression (rs = 0.571, P <0.05) <0.05). Conclusion The expression of K-ras protein and CyclinE in rectal cancer tissues is increased and the expression of p33ING1 protein is decreased. It is suggested that K-ras, p33ING1 and CyclinE play an important role in the carcinogenesis and progression of rectal cancer. And may provide a new method for the early diagnosis and treatment of rectal cancer.