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目的探讨甘精胰岛素、门冬胰岛素早期强化治疗发病非肥胖糖尿病(NOD)小鼠的可行性。方法选取近期发病NOD小鼠30只,随机平均分为A、B、C、D、E组。A、B、C、D组自由进食,每日9:00分别皮下注射甘精胰岛素0.1IU/g、0.05IU/g、0.015IU/g以及PBS5μL;E组给予胰岛素强化治疗:每日9:00皮下注射甘精胰岛素,并平均分6次予以食物1h,进食前皮下注射门冬胰岛素。同时选取同周龄未发病组小鼠为F组。观察各组治疗第4天24h血糖变化及第15天疗效。结果 E组小鼠24h中血糖均值显著低于B、C、D组(P≤0.05),与A、F组相比,差异无统计学意义(P>0.05),E组血糖波动范围较A、B、C组均显著减低(P<0.05)。E组24h中正常血糖所占的比例较B、C、D组均显著升高(P<0.05)。15d时A、B、C、E组糖尿病症状均得到控制,但A组有5只、B组有3只出现小鼠低血糖死亡。结论采用甘精胰岛素和门冬胰岛素联合强化治疗,其疗效优于单独应用甘精胰岛素各组。
Objective To investigate the feasibility of early intensive treatment of non-obese diabetic (NOD) mice with glargine and insulin aspart. Methods Thirty NOD-prone mice were randomly divided into A, B, C, D and E groups. Groups A, B, C and D were fed with insulin glargine at 0.1 IU / g, 0.05 IU / g, 0.015 IU / g and PBS 5 μL daily at 9:00 a.m. Group E received insulin intensive treatment: 00 subcutaneous injection of insulin glargine, and an average of 6 times to be food 1h, subcutaneous injection of insulin aspart before eating. At the same time select the same age non-onset group of mice for the F group. The changes of blood glucose on the 4th day of treatment and the curative effect on the 15th day were observed. Results The average blood glucose of mice in group E was significantly lower than that in groups B, C and D (P≤0.05), but there was no significant difference between groups A and F (P> 0.05) , B and C groups were significantly reduced (P <0.05). The proportion of normal blood glucose in 24h group was significantly higher than that in B, C and D groups (P <0.05). The symptoms of diabetes in groups A, B, C and E were all controlled on the 15th day, but there were 5 in the group A and 3 in the group B died of hypoglycemia. Conclusions The combination of insulin glargine and aspart insulin intensified treatment is superior to glargine alone.