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建立了高效液相色谱法测定中药材阳春砂中黄曲霉素(AFT)B_1、B_2、G_1、G_2的方法。样品经提取、免疫亲和层析净化,采用荧光检测器,以柱后衍生系统结合HPLC检测。结果表明,阳春砂中4种黄曲霉素在1.00 μg/kg,10.00μg/kg和32.00 μg/kg 3个水平的加标回收率均在84.0%～101.0%之间,相对标准偏差均小于1.7%;方法最低检出限AFB1与AFG1为0.35 μg/kg,AFB2与AFG2为0.06 μg/kg;标准曲线良好,相关系数大于0.998 7。本方法可用于阳春砂中黄曲霉素的检验。
A method for the determination of aflatoxins (AFT) B_1, B_2, G_1, G_2 in Chinese spring saplings was established by high performance liquid chromatography. Samples were extracted and purified by immunoaffinity chromatography using a fluorescence detector combined with post-column derivatization with HPLC. The results showed that the spiked recoveries of four aflatoxins at the concentrations of 1.00 μg / kg, 10.00 μg / kg and 32.00 μg / kg were all in the range of 84.0% -101.0% with relative standard deviations less than 1.7%. The minimum detectable limit of detection was 0.35 μg / kg for AFB1 and AFG1, and 0.06 μg / kg for AFB2 and AFB2. The calibration curve was good with the correlation coefficient greater than 0.998 7. The method can be used for checking aflatoxin in spring sand.