本实验采用酶方法在核苷酸水平上检测了8—MOP(8—methoxypsoian)诱导的四膜虫rDNA键间交联的产生。将四膜虫用不同浓度的8—MOP与365nm紫外线照射联合处理,提取其rDNA,再用限制性核酸内切酶ClaI和BamHI消化,经凝胶电泳,Southern转印,分子杂交,自显影等过程,获得最终结果。放射自显影图片显示,当8—MOP浓度≥1μg时,四膜虫rDNA分子产生交联,且随着8—MOP浓度的增加,链间交联的的分子数目也增加,呈现明显的剂量依赖关系。 In this study, 8-methoxypsozeine-induced cross-linking of tetradisia rDNA was examined at the nucleotide level by enzyme method. Tetrahymena with different concentrations of 8-MOP and 365nm UV irradiation combined treatment, extraction of rDNA, and then digested with restriction endonucleases ClaI and BamHI, by gel electrophoresis, Southern blotting, molecular hybridization, autoradiography Process, get the final result. Autoradiogram showed that tetracycline rDNA molecules were cross-linked when the concentration of 8-MOP was more than or equal to 1μg, and with the increase of 8-MOP concentration, the number of intermolecular cross-linked molecules also increased, showing a significant dose-dependent relationship.