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用聚丙烯酰胺凝胶电泳法对国内外不同地区来源的831个栽培粟地方品种进行了酯酶同工酶分析。所得酶谱可划分为13个表型。由品种来源地区和地区内各种表型的频率分布来研究各酶谱表型的地理分布。通过酯酶同工酶酶谱表型的遗传多样性分析探讨各地区品种的亲缘远近,比较栽培种与近缘野生种青狗尾草的酶谱表型来论证栽培粟的起源。初步查明欧洲粟地方品种酯酶同工酶表型分布和我国有较大差异。本项研究结果和新近国际文献有关粟同工酶、起源、分类的论述,许多观点是很接近的。分析结果表明,酯酶同工酶表型可以作为研究粟品种的亲缘关系、起源演变的一个生化指标。
Esterase isozymes were analyzed by polyacrylamide gel electrophoresis on 831 cultivated millet landraces from different regions at home and abroad. The resulting zymogram can be divided into 13 phenotypes. The geographical distribution of the phenotypic phenotypes was studied by the frequency distribution of various phenotypes in the regions and regions of origin of the cultivars. Through genetic diversity analysis of esterase isozymes phenotype, we discussed the genetic relationship of cultivars and wild relatives of Setaria viridis to prove the origin of cultivated millet. Preliminary identification of European millet varieties of esterase isozymes phenotype distribution and our country there is a big difference. The results of this study and the recent international literature on the millet isozyme, origin, classification of exposition, many views are very close. The results showed that the esterase isoenzyme phenotype can be used as a biochemical index to study the genetic relationship and origin of the millet varieties.