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目的:探讨血小板活化因子(PAF)、肿瘤坏死因子-α(TNF-α)及血小板源生长因子(PDGF)水平变化在毛细支气管炎并急性心衰的变化,探讨它们在该病的发病中的作用方法:58例心衰组患儿分别于治疗前和临床症状消失后采用双抗体夹心ABC-ELISA法检测PAF、TNF-α及PDGF血清含量,设正常对照组40例比较。结果:心衰组PAF治疗前为370.57±23.6ng/L,明显高于对照组的56.78±19.6ng/L,组间比较差异具有非常显著性意义(t=15.95,P<0.001);治疗后恢复至49.63±14.5 ng/L,与正常对照差异不明显;治疗前后比较,差异具有非常显著性意义(t=3.70,P<0.001)TNF-α治疗前为457.4±40.5 ng/L,明显高于对照组的148.8±21.6ng/L,组间比较差异具有显著性意义(t=2.135,P<0.05);治疗后恢复至162.6±37.61ng/L,与正常对照差异不明显;治疗前后比较,差异具有非常显著性意义(t=7.25,P<0.01)。PDGF治疗前为596.23±199.43)ng/L,较对照组259.76±69.58ng/L增高,组间比较,差异具有非常显著性意义(t=25.52,P<0.01);治疗后降为272.83±116.96ng/L,较治疗前明显恢复,组间比较差异均具有显著性意义(t=7.66,P<0.01)结论:结果表明,PAF、TNF-α、PDGF作为炎症介质不仅参与心衰的发病过程,还提示细胞外基质的异常在心衰发病学中的重要地位
Objective: To investigate the changes of platelet activating factor (PAF), tumor necrosis factor-α (TNF-α) and platelet-derived growth factor (PDGF) levels in patients with bronchiolitis and acute heart failure, and to explore their role in the pathogenesis of the disease Methods: The serum levels of PAF, TNF-α and PDGF in 58 patients with heart failure were detected by ABC-ELISA before treatment and after disappearance of clinical symptoms respectively. Forty cases in normal control group were compared. Results: Before treatment PAF was 370.57 ± 23.6ng / L in heart failure group, which was significantly higher than 56.78 ± 19.6ng / L in control group (t = 15.95, P <0.001) The difference was statistically significant (t = 3.70, P <0.001) before treatment and 457.4 ± 40.5 ng / L, which was significantly higher than that before treatment There was significant difference between the two groups (t = 2.135, P <0.05). After treatment, it recovered to 162.6 ± 37.61ng / L, which was not significantly different from the normal control group , The difference was very significant (t = 7.25, P <0.01). The level of PDGF was 596.23 ± 199.43 ng / L before treatment, which was significantly higher than that of control group (259.76 ± 69.58ng / L). There was a significant difference between the two groups (t = 25.52, P <0.01) (t = 7.66, P <0.01). CONCLUSION: PAF, TNF-α and PDGF are not only involved in the pathogenesis of heart failure, but also play an important role in the pathogenesis of heart failure , Also prompted the abnormalities of extracellular matrix in the pathogenesis of HF