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用7个同核异质不育系及其保持系,分别与2个恢复系配组成14个aF_1和2个bF_1为材料,测定了它们花药中17种结合氨基酸的含量。结果表明:1.凡育性可被恢复的不育系,花药中的天冬氨酸和蛋氨酸的含量相当于保持系的2.7～3.8倍和1.3～2.0倍,其它氨基酸的含量,除野汕A的胱氨酸外,则均低于保持系,相当于保持系的9.2%～87.5%之间。2.育性不能被恢复的隆汕A,花药中除天冬氨酸的含量高于保持系,其它氨基酸均低于保持系,但其中有12种氨基酸的含量较明显地高于育性可被恢复的aF_1。3.花药中脯氨酸、胱氨酸、蛋氨酸是和雄配子育性转换密切相关的3种氨基酸,当其含量偏低甚至缺失时,则雄配子败育,反之,当其恢复到接近正常含量时,则败育的雄配子转换为可育。
Seven isoforms of heterogenous male sterile lines and their maintainer lines were used to establish 14 aF_1 and two bF_1 lines with two restorer lines respectively. The contents of 17 kinds of bound amino acids in their anthers were determined. The results showed that: 1.As for fertility, the content of aspartic acid and methionine in anthers was 2.7-3.8 folds and 1.3-2.0 folds higher than those in maintainer lines. The contents of other amino acids except wild rice A outside of cystine, were lower than the maintainer line, equivalent to maintaining the line between 9.2% ~ 87.5%. 2. Fertility can not be restored in Longshan A, anther aspartic acid content than the maintainer, other amino acids were lower than the maintainer, but of which 12 kinds of amino acids were significantly higher than fertility Was restored aF_1.3. The anther proline, cystine, methionine and male gametes fertility is closely related to the conversion of three kinds of amino acids, when its content is low or even missing, then male gametes abortion, on the contrary, when its When it returns to normal levels, the aborted male gamete is converted to fertile.