大部分鲜食番茄及越来越多的加工番茄,都用杂种一代种子来生产。生产杂种种子的一般和有效方法,是通过细胞质雄性不孕性。不幸的是,雄性不孕体系在番茄上还未被开发,因此大部分杂种种子都是通过手工去雄和授粉而获得的,这使得杂种种子的价格很高。在番茄上存在着若干种核雄性不孕基因,这有可能利用于杂种种子的生产上,但有两个不利因素。其一,既然雄性不孕是隐性性状,那么把这种基因转入所期望的育种品系时很费事。在分离的群体中只有纯合子基因型才能被识别。在利用回交法来进行系内雄性不孕基因转移时,不可能把异合子基因型与纯合子基因型区别开。因此要求在两次回交的世代间进行后代检测;其二,这种基因一旦参入作为杂种种子生产亲本的染色体中,它将继续分 Most fresh tomatoes and more processed tomatoes are produced from the hybrid seed. The general and effective way to produce hybrid seeds is through cytoplasmic male sterility. Unfortunately, male infertility systems have not been developed on tomatoes, so most of the hybrid seeds are obtained by hand removal and pollination, which makes hybrid seeds expensive. There are several nuclear male sterility genes present in tomato, which are likely to be used in the production of hybrid seeds, but have two disadvantages. First, since male infertility is a recessive trait, it can be cumbersome to transfer this gene into the desired breeding line. Only homozygous genotypes can be identified in isolated populations. In the use of backcross method for in-line male infertility gene transfer, it is impossible to distinguish the heterozygous genotype and homozygous genotypes. It is therefore demanded that offspring be tested between the two backcrossed generations; and secondly, once this gene has been incorporated into the chromosome of a parent producing hybrid seeds, it will continue to be subdivided