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目的：比较破骨细胞与骨巨细胞瘤中多核巨细胞的特点，明确后者的性质和来源。方法：用倒置相差显微镜观察体外培养的多核巨细胞的一般形态及降钙素对它的影响；用骨片与多核巨细胞共同培养法观察多核巨细胞的体外骨吸收功能，用扫描电镜观察骨吸收陷窝，Ｇｏｍｏｒｉ染色观察多核巨细胞的酸性磷酸酶活性。结果：倒置相差显微镜下可见多核巨细胞胞核较多（２０个以上），胞浆周边不规则，有伪足样突起；胞浆内可见较多大小不等的空泡；降钙素（１００μｇ·Ｌ－１）可抑制多核巨细胞的伪足样运动；多核巨细胞与灭活的骨片共同培养时可见骨吸收陷窝形成，扫描电镜下可见吸收陷窝底面有原纤维；Ｇｏｍｏｒｉ染色时可见多核巨细胞的酸性磷酸酶呈阳性。结论：证实了多核巨细胞具有破骨细胞的形态特征与骨吸收功能，可能来源于骨髓的破骨细胞前体细胞
OBJECTIVE: To compare the characteristics of multinucleated giant cells in osteoclasts and giant cell tumors of bone, and to clarify the nature and origin of the latter. Methods: Inverted phase contrast microscopy was used to observe the general morphology of multinucleated giant cells cultured in vitro and the effect of calcitonin on it. The bone marrow and multinucleated giant cells co-culture method was used to observe the bone resorption function of multinucleated giant cells in vitro. The bone was observed by scanning electron microscope. The lacunae were absorbed and Gomori staining was used to observe the acid phosphatase activity of multinucleated giant cells. RESULTS: Inverted phase contrast microscopy showed that there were more nuclei in multinucleated giant cells (20 or more), irregular peripheral cytoplasm, and pseudopod-like processes; more vacuoles of different sizes were seen in the cytoplasm; calcitonin (100 μg) L-1) can inhibit pseudopox-like movement of multinucleated giant cells; bone resorption lacuna formation can be seen when multinucleated giant cells co-culture with inactivated bone fragments; fibrils can be seen underneath the absorption lacunae under scanning electron microscope; when Gomori stains It can be seen that the multinucleated giant cells are positive for acid phosphatase. Conclusion: It was confirmed that multinucleated giant cells have the morphological characteristics and bone resorption of osteoclasts, which may be derived from the osteoclast precursor cells of the bone marrow.