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目的分析6株恶性疟原虫PfCP-2.9融合抗原的单克隆抗体识别相同或相关表位情况。方法以PfCP-2.9重组蛋白为抗原进行ELISA板包被,用辣根过氧化物酶分别标记6株PfCP-2.9单克隆抗体,并以此作为竞争检测抗体,与这6株未标记的单抗进行相互之间的竞争检测。结果根据竞争ELISA结果可将6株单抗分为2组,2组单抗之间无竞争,而在各组内的单克隆抗体发生相互之间的竞争。结论存在相互竞争的单抗可能识别同一表位或相关表位,研究结果为表明这些单抗识别特性提供信息。
Objective To analyze the identity of the same or related epitopes by monoclonal antibodies against six P. falciparum PfCP-2.9 fusion antigens. Methods PfCP-2.9 recombinant protein was used as antigen to coat ELISA plate, and 6 monoclonal antibodies against PfCP-2.9 were respectively labeled with horseradish peroxidase (HRP). The monoclonal antibodies were used as competitive detection antibodies, and these 6 unlabeled monoclonal antibodies Conduct mutual competition testing. Results According to the competitive ELISA results, the six monoclonal antibodies were divided into two groups. There was no competition between the two monoclonal antibodies, and the monoclonal antibodies in each group competed with each other. CONCLUSIONS: The presence of competing monoclonal antibodies may recognize the same epitope or related epitopes and the results provide information to identify the identity of these mAbs.