对改性聚酰胺吸附层析柱进行了系统的研究，利用β萘酚橙获得了该柱一系列有关信息，如：最佳聚酰胺涂装量，水洗最佳流速范围，最小水洗用量，饱和吸附量。得出了β萘酚橙在该层析柱上的吸附为单分子层吸附的结论。使用本法在银杏叶黄酮的分离中得到了总黄酮含量超过５０％的满意结果。 A systematic study on the modified polyamide adsorption column was carried out. A series of relevant information about the column was obtained using β-naphthol orange, such as the optimal polyamide coating amount, the optimum flow rate range for washing, the minimum water washing amount, Saturated adsorption capacity. The conclusion is that the adsorption of β-naphthol orange on the column is the monolayer adsorption. Use this method in the separation of flavonoids Ginkgo biloba obtained a total flavonoid content of more than 50% of the satisfactory results.