论文部分内容阅读
采用 4 18个引物对尼罗罗非鱼进行了RAPD分析 ,筛选出 4 6个重复性好、可用于尼罗罗非鱼RAPD标记的引物 .另用 12个引物对尼罗罗非鱼群体 (2 3尾 )进行了遗传多样性分析 :共检出 5 8个位点 ,其中 18个位点表现出多态性 ,多态位点比例达 31.0 3% ,其群体的Nei&Li氏遗传相似率为 90 .2 3% ,申农信息指数为0 .10 0 5bit.上述三项指标表明 ,该尼罗罗非鱼群体保持着较高水平的遗传变异 .
A total of 46 primers were used to detect the RAPD markers of Nile tilapia (Nile tilapia) by using RAPD analysis with 4 18 primers, 2 3) were analyzed by genetic diversity analysis. A total of 58 sites were detected, of which 18 loci showed polymorphism and the percentage of polymorphic loci was 31.0 3%. The genetic similarity of Nei & Li population was 90.23% and Shennong Information Index is 0.1050bit.The above three indexes indicated that the Nile tilapia population maintained a high level of genetic variation.