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目的:通过用脂质体包裹靶向局部粘着斑激酶(focal adhesion kinase,FAK)基因的短发夹RNA(short hairpin RNA,shRNA)质粒(shRNA-FAK),探讨该方法对荷瘤裸鼠中人肺腺癌SPC-A1的抑制效果。方法:用脂质体包裹shRNA-FAK,并通过尾静脉注射治疗荷瘤裸鼠,通过肿瘤生长曲线和重量,肿瘤组织形态学,肿瘤中的FAK蛋白表达情况、CD31、VEGF、PCNA和TUNEL检测,分析该方法对荷瘤裸鼠中人肺腺癌SPC-A1的抑制效果。结果:荷瘤裸鼠经过脂质体shRNA-FAK治疗后,肿瘤生长速度明显缓于对照组(P<0.05),肿瘤重量明显低于对照组(P<0.05)。肿瘤组织的免疫组化、western blot和TUNEL检测显示与对照组相比,治疗组FAK蛋白表达降低(P<0.05)、血管生成和细胞增殖均减少(P<0.05)、凋亡细胞增加(P<0.05),荷瘤裸鼠主要脏器形态学正常。结论:FAK特异性的小干扰RNA(small interfering RNA,siRNA)能够在体内阻断FAK蛋白的表达,使组织内FAK蛋白的表达相应地减少,并进一步诱导肿瘤细胞的凋亡,最终导致肿瘤的生长速度减缓。
OBJECTIVE: To investigate the effect of this method on the expression of FAK gene in hairless nude mice by using liposomes to coat short hairpin RNA (shRNA) plasmid (shRNA-FAK) targeting focal adhesion kinase (FAK) Inhibitory effect of SPC-A1 on human lung adenocarcinoma. Methods: The shRNA-FAK was encapsulated by liposomes, and the tumor-bearing nude mice were treated by tail vein injection. The tumor growth curve and weight, tumor histomorphology, FAK protein expression, CD31, VEGF, PCNA and TUNEL The inhibitory effect of this method on human lung adenocarcinoma SPC-A1 in tumor-bearing nude mice was analyzed. Results: The tumor growth rate of tumor-bearing nude mice treated with liposome shRNA-FAK was significantly slower than that of the control group (P <0.05). The tumor weight was significantly lower than that of the control group (P <0.05). Immunohistochemistry, western blot and TUNEL showed that the expression of FAK protein in the treatment group decreased (P <0.05), angiogenesis and cell proliferation decreased (P <0.05) and the apoptotic cells increased (P <0.05), tumor-bearing nude mice morphology of normal organs. CONCLUSIONS: FAK-specific small interfering RNA (siRNA) blocks the expression of FAK protein in vivo and accordingly reduces the expression of FAK protein in the tissue and further induces the apoptosis of tumor cells, which eventually leads to tumor Slow down.