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目的:研究福州市2005年霍乱菌株DNA分子特征,分析其同源性及流行病学意义。方法:采用聚合酶链反应(PCR)及随机引物多态性扩增(RAPD)等分子生物学技术对菌株进行检测,研究本地菌株的分子特征,RAPD结果用SAS9.0软件进行多态性比较。结果:对40株霍乱弧菌4种毒力基因检测结果表明,39株霍乱弧菌均检出ctxA、tcpA、ace、zot 4种毒力基因,1株外环境菌株4种毒力基因均为阴性。40株霍乱弧茵RAPD结果经聚类分析可分为4个聚类群,其中37株属于同一聚类型别,另3株分别属于不同聚类型。结论:福州市2005年流行菌株以稻叶型占绝对优势,流行优势菌株分子遗传特征具有高度的同源性。本次研究采用的PCR及RAPD等分子生物学方法在霍乱流行病学调查中具有较高的实用价值。
Objective: To study the molecular characteristics of DNA in cholera strains in Fuzhou in 2005, and analyze their homology and epidemiological significance. Methods: The strains were detected by polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD) and other molecular biological techniques to study the molecular characteristics of local strains. The results of RAPD were compared using SAS9.0 software . Results: The results of four virulence genes of 40 V. cholerae strains showed that all the 39 strains of V. cholerae detected four virulence genes of ctxA, tcpA, ace and zot, and one of the four virulence genes of one strain negative. The 40 strains of V. cholerae arc RAPD results can be divided into four clusters by cluster analysis, of which 37 belong to the same cluster type, and the other three belong to different cluster types. Conclusion: In 2005, endemic strains of rice in Fuzhou occupied absolute predominance of rice leaf type, and the molecular genetic characteristics of prevalent strains had high homology. The PCR and RAPD molecular biology methods used in this study have high practical value in epidemiological investigation of cholera.