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应用125Ⅰ标记放免法对比测定病区补硒(200μgSe/d,亚硒酸钠,12周)和未补硒居民血小板TXA2生成及血浆TXA2和前列环素(PGI2),用2,3一二氨基萘荧光法和谷胱甘肽还原酶偶联法测定硒含量和GSH-Px活性。结果表明:(1)补硒组红细胞硒和GSH-Px活性显著高于未补硒组(P<0.01);(2)补硒组和未补硒组血浆PGI2水平之间无显著差异(P>0.05);(3)补硒组血浆TXA2含量、TXA2/PGI2。比值和血小板TXA2生成均显著低于未补硒组(P<0.01);(4)血浆TXA2含量和血小板TXA2生成呈显著正相关(r=0.825,P<0.01);红细胞硒含量和GSH-Px活性分别与血浆TXA2含量或血小板TXA2生成呈显著负相关(r=-0.696,-O.780,-0.676,-0.796,P<0.01)。研究说明,给病区低硒居民补硒可减少其血小板TXA2生成,使血浆TXA2水平也下降,两者的降低与补硒后硒状态的增高有密切关系。
The 125I-labeled radioimmunoassay was used to determine the TXA2 production and TXA2 and PGI2 in sera of patients with selenium (200μgSe / d, sodium selenite, 12 weeks) Determination of Selenium Content and GSH-Px Activity by Naphthalene Fluorescence and Glutathione Reductase Coupling Methods. The results showed that: (1) The activities of selenium and GSH-Px in selenium group were significantly higher than those in selenium-deficient group (P <0.01); (2) There was no significant difference in plasma PGI2 level between selenium- (P> 0.05). (3) TXA2 content in selenium-supplemented group, TXA2 / PGI2. (P <0.01). (4) There was a significant positive correlation between plasma TXA2 level and platelet TXA2 production (r = 0.825, P <0.01) Selenium content and GSH-Px activity were negatively correlated with plasma TXA2 level or platelet TXA2 production (r = -0.696, -0.780, -0.676, -0.796, P <0.01). Studies have shown that low selenium residents to the ward to selenium can reduce the TXA2 platelet production, the plasma TXA2 levels also decline, the reduction of both are closely related to selenium status after selenium increase.