论文部分内容阅读
[目的 ] 探讨间接免疫荧光技术 (IFA)在柯萨奇B组病毒 (CBV)中枢神经系统感染中的早期诊断价值。[方法 ] 用IFA法检测 189例病毒性脑膜炎和脑炎患者在发病 1周内和发病 2～ 3周后的脑脊液和血清标本中CBV3和CVB5特异性IgG、IgM。 [结果 ] 脑脊液和血清标本中特异性IgM阳性率在发病 1周内分别为 13 9%和 4 5 %,脑脊液特异性IgM的阳性率显著高于血清标本 ( χ2 =3 97,P <0 0 5 ) ;发病 2～ 3周后 ,则分别为 14 5 %和 16 7%,两者阳性率差异无显著性 (Fisher精确概率P =0 80 4) ;脑脊液中特异性IgG阳性率比IgM高。 [结论 ] 用IFA法检测早期脑脊液中特异性IgM可用于早期快速诊断柯萨奇B组病毒中枢神经系统感染。
[Objective] To explore the value of indirect immunofluorescence (IFA) in the early diagnosis of Coxsackie virus B (CNV) infection in the central nervous system. [Method] The specific IgG and IgM of CBV3 and CVB5 in cerebrospinal fluid and serum samples of 189 patients with viral meningitis and encephalitis were detected by IFA in 1 week and 2 to 3 weeks after onset. [Results] The positive rates of specific IgM in cerebrospinal fluid (CSF) and serum samples were 13 9% and 45% within 1 week of onset, respectively. The positive rate of cerebrospinal fluid specific IgM was significantly higher than that of serum samples (χ2 = 3 97, P <0 0 5). After 2 to 3 weeks, the positive rate of CSF was 14 5% and 16 7% respectively (Fisher exact probability P = 0 80 4). The positive rate of specific IgG in cerebrospinal fluid was higher than that of IgM . [Conclusion] The detection of specific IgM in early cerebrospinal fluid by IFA can be used to diagnose the infection of Coxsackie B virus in the central nervous system in the early stage.