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胰岛β细胞分泌胰岛素(1)时,同时分泌少量的胰岛素原(PI)。我们设想加重刺激促使I分泌时,含有多量PI的未成熟颗粒可能出现在血液中,使血PI值和PI/I比值升高。我们应用PI特异性抗体建立了能简易地测定PI的RIA测定法,用以观察NIDDM的PI分泌情况的动态性变化。以rDNA人PI免疫豚鼠制成抗体。用氯胺T法(Chloramine T method)标识PI,以双抗体法分离BF。健康者15人,未治疗的非肥胖型NIDDM病人25人,他们各服100gOGTT后,按规定时间采血,作血糖、I和PI的测定。糖尿病病人按其空腹时血糖值(FBS,mg/dl)分为Ⅰ组(FBS<150),Ⅱ组(150≤FBS<200),Ⅲ组(200≤FBS<250),和Ⅳ组(FBS≥250)。
Pancreatic β cells secrete a small amount of proinsulin (PI) while secreting insulin (1). When we envisage aggravating the stimuli to promote the secretion of I, immature particles containing a large amount of PI may appear in the blood to increase the blood PI value and the PI / I ratio. Using PI-specific antibodies, we established an RIA assay that allows for the easy determination of PI for observing the dynamic changes in PI secretion of NIDDM. The guinea pigs were immunized with rDNA human PI to make antibodies. PI was identified by the Chloramine T method and BF was separated by the double antibody method. 15 healthy people and 25 untreated non-obese NIDDM patients. After taking 100gOGTT each time, they took blood for blood sugar, blood glucose, I and PI. Diabetic patients were divided into group I (FBS <150), group II (150≤FBS <200), group III (200≤FBS <250) and group FBS according to their fasting blood glucose (FBS, ≥250).