论文部分内容阅读
以那贺川野菊为试材,研究了不同浓度蔗糖和多效唑(PP333)对试管苗离体保存的影响,并对保存材料再生后代的遗传稳定性进行了分析。结果表明:常温(23±2)℃、光照强度2000~3000lx、光照时间12h·d-1的培养条件下,在MS+2.0mg·L-1KT+0.1mg·L-1NAA+6.5g·L-1琼脂培养基中,蔗糖浓度为15~30g·L-1时附加6~9mg·L-1PP333能保存试管苗360d以上,存活率达93.53%~100%,且恢复生长后试管苗长势良好,其再生后代的形态特征、过氧化物酶(POD)酶谱和ISSR-PCR扩增图谱与对照相比没有明显差异。
The effect of sucrose and paclobutrazol (PP333) on the in vitro survival of in vitro plantlets was studied in the paper, and the genetic stability of regenerated progenies of the preserved materials was analyzed. The results showed that under MS (2.0mg · L-1KT + 0.1mg · L-1NAA + 6.5g · L) culture at room temperature (23 ± 2) ℃, light intensity 2000 ~ 3000lx and illumination time 12h · d-1, -1 agar medium, sucrose concentration of 15 ~ 30g · L-1, additional 6 ~ 9mg · L-1PP333 can save more than 360 days in vitro seedlings, the survival rate of 93.53% to 100%, and the growth of test tube plantlets after good growth The morphological characteristics, peroxidase (POD) and ISSR-PCR amplification patterns of regenerated progenies showed no significant difference compared with the control.