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目的 分析 33 5× 10 3 泡蛋白促成核活性以及多肽、糖链改变对其活性的影响。 方法 制备人工模拟胆汁 ,在偏光显微镜下观察结晶成核生长过程 ,计算成核活性 ;酶法水解蛋白多肽与糖链结构 ,通过结晶生长试验检测 33 5× 10 3 泡蛋白促结晶生长作用以及糖链水解、多肽降解产物的活性改变。 结果 33 5× 10 3 泡蛋白具有强促成核活性 ,成核活性强达 0 310 ,在不同模拟胆汁体系中 ,成核活性基本相同 ;其结晶生长试验活性指数It、Ig、Ic分别为 0 5 7、1 5 2及 1 6 3(P <0 0 5 )。糖苷酶切、肽链酶解后其成核活性均几乎丧失。 结论 33 5× 10 3 泡蛋白可能参与成石过程 ,其中多肽、糖链调节其成核活性。
OBJECTIVE: To analyze the effects of 33 5 × 10 3 vacuolar proteins on nuclear activity, changes of polypeptide and sugar chains. Methods Artificial simulated bile was prepared and the nucleation and growth of nuclei were observed under polarized light microscope to calculate the nucleation activity. The enzymatic hydrolysis of protein and sugar chain structure was carried out. The crystal growth of 33 5 × 10 3 vesicles was detected by crystal growth assay. Chain hydrolysis, changes in the activity of polypeptide degradation products. Results 33 5 × 10 3 vacuolar proteins had strong nucleophilic nucleation activity, strong nucleation activity of 0 310, and nucleophilic activity was basically the same in different simulated bile systems. Its crystal growth activity index (It, Ig, Ic) was 0 5 7,1 5 2 and 1 6 3 (P <0 0 5). Glycosidase digestion, peptide chain enzymatic activity were almost lost its nucleation. Conclusion 33 5 × 10 3 bulbs may participate in the process of stone formation, in which the polypeptide and sugar chain regulates their nucleation activity.