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单纯疱疹病毒(HSV)是人的重要的致病性病毒,能引起原发性感染、复发性感染、潜伏性感染和先天性感染,甚至与癌肿的发生也有关联。随着核酸分子杂交,限制性内切酶酶解分析,核酸序列分析,病毒DNA片段的克隆化等新技术不断地发明和日益广泛地应用,使得人们对HSV DNA的结构和功能及其与宿主细胞的关系有了更深入的认识。HSV DNA的理化性质和结构特点1.HSV DNA的理化性质HSV DNA是一种线型双股分子,分子量(96±5)×10~6。HSV-2DNA的分子量比HSV-1 DNA的分子量大3×10~6。HSV-1和2DNA序列分别含有67％和69％的G+C。两者的同源性约为50％。限制性内切酶酶解分析表明,同一血
Herpes simplex virus (HSV) is an important human pathogenic virus, can cause primary infection, recurrent infection, latent infection and congenital infection, even with the occurrence of cancer are also related. With the hybridization of nucleic acid molecules, restriction enzyme digestion analysis, nucleic acid sequence analysis, virus DNA fragment cloning and other new technologies continue to be invented and increasingly widely used, making the structure and function of HSV DNA and its host and host Cell relationship has a deeper understanding. Physical and Chemical Properties and Structural Features of HSV DNA 1. Physical and Chemical Properties of HSV DNA HSV DNA is a linear double-stranded molecule with a molecular weight of (96 ± 5) × 10 ~ 6. The molecular weight of HSV-2 DNA is 3 × 10 ~ 6 higher than that of HSV-1 DNA. The HSV-1 and 2 DNA sequences contain 67% and 69% G + C, respectively. The homology between the two is about 50%. Restriction enzyme digestion analysis showed that the same blood