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目的:探讨结肠癌组织中UHRF1、Ki-67蛋白的表达意义及其预测结肠癌患者预后的价值。方法:采用免疫组织化学法检测70例结肠癌组织和30例癌旁正常组织中UHRF1、Ki-67蛋白的表达情况,分析两者在结肠癌发生发展中相关性,及其与结肠癌患者临床病理特征和预后的关系。结果:UHRF1和Ki-67在癌组织中表达均明显高于癌旁正常结肠组织(P<0.05),且两者在结肠癌中呈正相关。UHRF1、Ki-67蛋白在结肠癌中表达与肿瘤TNM分期、浸润深度及淋巴结转移密切相关(P<0.05),与患者年龄、性别、肿瘤大小、分化状态无关(P>0.05)。UHRF1、Ki-67均阳性表达患者的3年生存率和中位生存时间较单独UHRF1阳性表达患者低(P>0.05),且明显较UHRF1、Ki-67阴性表达者低(P<0.05)。结论:UHRF1、Ki-67与结肠癌的发生发展密切相关,二者的表达强度可以作为患者预后的重要指标。
Objective: To investigate the significance of expression of UHRF1 and Ki-67 in colon cancer and their prognostic value in patients with colon cancer. Methods: Immunohistochemistry was used to detect the expression of UHRF1 and Ki-67 in 70 cases of colorectal cancer tissue and 30 cases of adjacent normal tissues. The correlation between the expression of UHRF1 and Ki-67 in colorectal carcinomas was analyzed. Relationship between pathological features and prognosis. Results: The expression of UHRF1 and Ki-67 in carcinoma tissue was significantly higher than that in adjacent normal colorectal tissues (P <0.05), and both were positively correlated in colon cancer. The expression of UHRF1 and Ki-67 in colon cancer was closely related to TNM stage, depth of invasion and lymph node metastasis (P <0.05), but not to age, sex, tumor size and differentiation (P> 0.05). The 3-year survival rate and median survival time of patients with positive expression of UHRF1 and Ki-67 were significantly lower than those of patients with UHRF1 alone (P> 0.05), and were significantly lower than those with negative expression of UHRF1 and Ki-67 (P <0.05). Conclusion: The expression of UHRF1 and Ki-67 is closely related to the occurrence and development of colon cancer. The expression intensity of both can be used as an important index of prognosis in patients.