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在成功克隆人肝再生增强因子（hALR）CDNA的基础上，本研究将人ALR编码区cDNA亚克隆于真核瞬间表达质粒pCDNAⅠ，构建了真核表达质粒pCDNAⅠ－hALR，转染cos-7细胞后，表达产物生物活性检测表明：真核表达的hALR在体外能刺激HTC肝癌细胞增殖，这一体外活性的确定为重组人ALR的纯化提供了简洁、可靠的检测方法。
After successful cloning of human liver regeneration enhancing factor (hALR) CDNA, the human ALR coding region cDNA was subcloned into the eukaryotic transient expression plasmid pCDNAI, and the eukaryotic expression plasmid pCDNAI-hALR was constructed and transfected into cos-7 cells. After the detection of the biological activity of the expressed product, the eukaryotic expression of hALR could stimulate the proliferation of HTC hepatoma cells in vitro. This in vitro activity determination provides a simple and reliable detection method for the purification of recombinant human ALR.