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目的探讨扶正养肝方对急性免疫性肝损伤抗肝细胞凋亡及分子机制,为其临床应用及院内制剂的开发奠定基础。方法 40只昆明种小鼠,随机分为4组,即正常对照组、病理模型组、联苯双酯滴丸组、扶正养肝方组。建立刀豆蛋白(Concanavalin,Con A)诱导免疫性肝损伤模型,检测各组小鼠血清AST水平,石蜡切片HE染色测定肝脏病理损伤程度。TUNEL法检测肝细胞凋亡,实时荧光定量PCR法检测肝损伤模型小鼠Fas和Survivin mRNA表达水平。结果扶正养肝方药物干预显著降低血清AST水平(P<0.05),改善小鼠肝脏病理损伤程度,抑制肝损伤模型小鼠肝细胞凋亡(P<0.05)。同时,扶正养肝方干预后显著下调Fas mRNA表达水平(P<0.01),然而对Survivin mRNA基因表达水平无明显影响(P>0.05)。结论扶正养肝方对急性免疫性肝损伤具有保护作用,其作用机制可能与下调Fas mRNA基因表达,抑制肝细胞凋亡有关。
Objective To explore the molecular mechanism of Fuzheng Yanggan on acute hepatic injury induced by acute liver injury and to lay the foundation for its clinical application and the development of in-hospital preparations. Methods Forty Kunming mice were randomly divided into 4 groups: normal control group, pathological model group, bifendate drip group, and Fuzheng Yanggan Fang group. A model of immune liver injury induced by Concanavalin (Con A) was established. The level of serum AST in each group was detected. The degree of hepatic pathological injury was determined by HE staining. TUNEL assay was used to detect hepatocyte apoptosis. Real-time fluorescence quantitative PCR was used to detect the expression of Fas and Survivin mRNA in liver injury model mice. Results Fuzheng Yanggan Decoction significantly decreased the level of serum AST (P <0.05), ameliorated the degree of hepatic pathological injury in mice, and inhibited the hepatocyte apoptosis in liver injury model mice (P <0.05). At the same time, Fas mRNA expression was significantly down-regulated by Fuzheng Yanggan Decoction (P <0.01), however, no significant effect was found on Survivin mRNA expression (P> 0.05). Conclusion Fuzheng Yanggan Decoction has a protective effect on acute immunological liver injury. Its mechanism may be related to the down-regulation of Fas mRNA gene expression and the inhibition of hepatocyte apoptosis.