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目的分析福建省2010-2011年手足口病患者中肠道病毒71型(EV71)分离株的基因特征。方法从福建省2010年和2011年手足口病患者标本中分离到EV71病毒74株(2010年50株,2011年24株),用RT-PCR法扩增VP1区并测序,利用DNAStar和MEGA4.0分析软件进行同源性分析和构建系统发生树。结果 74株病毒的核苷酸和氨基酸序列同源性分别为95.4%～100.0%和97.3%～100.0%;与安徽省阜阳市2008年流行株Fuyang17.08-2的核苷酸和氨基酸序列同源性分别为97.1%～99.3%和98.7%～100.0%,在系统进化树上与C4a亚型代表株处于同一分支,属于C4a基因亚型。2010年分离的4株EV71与阜阳代表株在SP70区域的氨基酸序列存在差异,而SP55区域的氨基酸序列则完全一致。结论福建省2010年和2011年手足口病患者中EV71的主要流行型别为C4a亚型,处于不同的进化链中,提示福建省可能存在多个传播链及部分EV71分离株具有进化趋势,对EV71的分子流行病学监测和分析有助于揭示病毒传播及进化规律。
Objective To analyze the genetic characteristics of Enterovirus 71 (EV71) isolates from HFMD in Fujian Province from 2010 to 2011. Methods 74 strains of EV71 virus (50 strains in 2010 and 24 strains in 2011) were isolated from specimens of HFMD patients in 2010 and 2011 in Fujian Province. The VP1 region was amplified by RT-PCR and sequenced. DNAStar and MEGA4 were used. 0 analysis software for homology analysis and phylogenetic tree construction. Results The nucleotide and amino acid sequence identities of 74 strains of viruses were 95.4% -100.0% and 97.3% -100.0%, respectively. The nucleotide and amino acid sequences of Fuyang17.08-2 were identical with those of Fuyang city in 2008 The genotypes were from 97.1% to 99.3% and from 98.7% to 100.0%, respectively, which belonged to the C4a subtype on the phylogenetic tree with the C4a subtype representative strain. There were differences in the amino acid sequences of the four strains of EV71 isolated from the Fuyang strain in the SP70 region in 2010, while the amino acid sequences of the SP55 region were completely identical. Conclusion The prevalence of EV71 in HFMD patients in Fujian Province in 2010 and 2011 is C4a subtypes, which are in different evolutionary chains, suggesting that there may be multiple transmission chains and some EV71 isolates in Fujian Province. The molecular epidemiological surveillance and analysis of EV71 help to reveal the regularity of virus transmission and evolution.