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目的:观察地塞米松对哮喘豚鼠血清白细胞介素-4(IL-4)及肺组织形态的影响,探讨糖皮质激素治疗哮喘的机制。方法:30只健康雄性Hartley系豚鼠随机分为空白对照组,病理模型组,地塞米松组。用卵白蛋白(OVA)复制哮喘豚鼠模型。采用流式细胞术分析CD4+interleukin-4(IL-4)细胞占CD4+T细胞的比例,光镜检查肺组织形态变化。结果:病理模型组外周血中IL-4明显高于空白对照组(P<0.05),地塞米松组外周血中IL-4较病理模型组明显降低(P<0.05),且地塞米松组支气管上皮损伤,粘液腺增生等病理改变较病理模型组明显改善。结论:地塞米松治疗哮喘的作用与抑制Th2的活化,改善支气管上皮损伤,粘液腺增生等病理改变有关。
Objective: To observe the effects of dexamethasone on serum interleukin-4 (IL-4) and lung morphology in asthmatic guinea pigs and explore the mechanism of glucocorticoid treatment of asthma. Methods: Thirty healthy male Hartley guinea pigs were randomly divided into blank control group, pathological model group and dexamethasone group. Asthma guinea pig models were replicated with ovalbumin (OVA). Flow cytometry was used to analyze the proportion of CD4 + T cells in CD4 + T cells. The morphological changes of lung tissue were examined by light microscopy. Results: The level of IL-4 in the peripheral blood of the pathological model group was significantly higher than that of the blank control group (P <0.05), while the level of IL-4 in the dexamethasone group was significantly lower than that of the pathological model group (P <0.05) Bronchial epithelial injury, mucinous gland hyperplasia and other pathological changes than the pathological model group was significantly improved. Conclusion: The effect of dexamethasone on asthma is related to inhibiting the activation of Th2 and improving the pathological changes of bronchial epithelial injury and mucinous gland hyperplasia.