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用大量食蟹猴疟原虫子孢子(2.1×10~6)和猪尾猴疟虫子孢子(9.53×10~6)各经股静脉接种一猕猴。前者在接种子孢子后第7天,后者在第8天分别出现首次原虫血症。两猴皆用氯喹(10mg/kg)连续5天作胃管灌服,以清除血中原虫。食蟹猴疟原虫经氯喹治疗后出现3次复发,时间分别为子孢子接种后30,56和155天。每次复发均用上述剂量的氯喹作同样的治疗。在猪尾猴疟原虫则在第1次氯喹治疗后一直无复发出现。用间接荧光抗体染色(IFAT)和吉氏染色法在食蟹猴疟原虫子孢子感染后第7天和62天的猴肝切片中均找到典型的休眠体,而在感染猪尾猴疟原虫第8天的肝切片中,只查到发育成熟的裂殖体而无休眠体。实验结果进一步证实,疟疾“复发”是“休眠体”经过一段时间蛰伏后发育成裂殖子所引起的。
A monkeys were inoculated via the femoral vein with a large number of malaria parasite sporozoites (2.1 × 10 -6) and pigs macaques (9.53 × 10 -6). The former on the 7th day after inoculation of sporozoites, the latter on the first 8 days of the first parasitemia. Both monkeys are using chloroquine (10mg / kg) for 5 consecutive days for gastric tube gavage to clear the parasite in blood. Plasmodium cynocephalus was treated with chloroquine three times after the relapse, the time was 30, 56 and 155 days after inoculation of sporozoites. Each recurrence with the above doses of chloroquine for the same treatment. In pigs, Plasmodium had no recurrence after the first chloroquine treatment. Indirect fluorescent antibody staining (IFAT) and Giemsa staining found typical dormant bodies in the liver slices on day 7 and day 62 after infection with Plasmodium malariae sporozoites, respectively. However, Days of liver slices, only to find the development of mature schizonts without resting body. The experimental results further confirmed that “relapse” of malaria is caused by the development of meiosis in the “dormant body” after a period of dormancy.