论文部分内容阅读
目的探讨丹参注射液体外对肝星状细胞HSC-T6的毒性及其作用机制。方法利用细胞培养技术,苔盼蓝法检测系列丹参溶液对HSC-T6生长的影响,Gimsa染色观察细胞形态,MTT法检测丹参对细胞的生长抑制率,免疫组化检测血小板衍生生长因子(PDGF-BB)表达,生物法检测转化生长因子的表达。结
Objective To investigate the toxicity and mechanism of Salvia miltiorrhiza injection on HSC-T6 in hepatic stellate cells. Methods Cell culture technique was used to study the effects of serial salviae miltiorrhizae solution on the growth of HSC-T6 cells. The morphology of cells was observed by Gimsa staining. The growth inhibitory rate of salvia miltiorrhiza cells was detected by MTT assay, and platelet-derived growth factor (PDGF-) was detected by immunohistochemistry. BB) expression, biological detection of transforming growth factor expression. Knot