提出一种简便的免疫亲和层析方法，有效地分离纯化重组人肿瘤坏死因子（ｒＨＴＮＦ），此法不需预先分离纯化单克隆抗体．用交联刘ｄｉｍｅｔｈｙｌｓｕｂｅｒｉｍｉｄａｔｅ使抗ＴＮＦ的单克隆重抗体和ｐｒｏｔｅｉｎＡ—ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ共价结合，成为稳定的免疫基质，制备免疫亲和层析柱，将重组ＴＮＦ粗制品一步纯化为均一组份． A simple immunoaffinity chromatography method was proposed to efficiently isolate and purify recombinant human tumor necrosis factor (rHTNF). This method does not require prior isolation and purification of monoclonal antibodies. The anti-TNF monoclonal antibody and protein A-Sepharose CL-4B were covalently linked by cross-linking with dimethylsuberimidate to form a stable immunological matrix. Immuno-affinity chromatography was performed and the crude recombinant TNF was purified in one step to homogeneity.