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目的:建立以L-半胱氨酸包覆ZnS纳米粒子为荧光探针测定药物制剂中诺氟沙星含量的荧光分析法。方法:研究了L-半胱氨酸包覆ZnS纳米粒子对诺氟沙星荧光强度的影响,发现在KH2PO4-NaOH缓冲溶液中,诺氟沙星与L-半胱氨酸包覆ZnS纳米粒子相互作用使体系的荧光强度增强。测定时最佳pH为6.5,L-半胱氨酸包覆ZnS纳米粒子的最佳浓度为4.0×10-4mol·L-1。结果:诺氟沙星浓度在4.0×10-9～1.2×10-6mol·L-1范围内与体系的荧光强度线性关系良好,r=0.9985,检出限(S/N=3)为7.4×10-10mol·L-1,定量限(S/N=10)为4.0×10-9mol·L-1。对浓度为1.6×10-7mol·L-1诺氟沙星标准溶液平行测定9次的RSD为1.8%。结论:该法简便、快速,灵敏度高,可用于直接测定诺氟沙星含量。
OBJECTIVE: To establish a fluorescence assay for the determination of norfloxacin in pharmaceutical preparations using L-cysteine-coated ZnS nanoparticles as fluorescent probes. Methods: The effect of L-cysteine coated ZnS nanoparticles on the fluorescence intensity of norfloxacin was studied. It was found that in the KH2PO4-NaOH buffer solution, norfloxacin and L-cysteine coated ZnS nanoparticles Interaction of the system to enhance the fluorescence intensity. The optimum pH was 6.5 and the optimal concentration of L-cysteine coated ZnS nanoparticles was 4.0 × 10-4 mol·L-1. Results: The concentration of norfloxacin in the range of 4.0 × 10-9 ~ 1.2 × 10-6mol·L-1 showed a good linear relationship with the fluorescence intensity of the system (r = 0.9985). The detection limit (S / N = 3) was 7.4 × 10-10mol·L-1, and the limit of quantitation (S / N = 10) was 4.0 × 10-9mol·L-1. The RSD of 9 parallel determinations of norfloxacin standard solution at a concentration of 1.6 × 10-7mol·L-1 was 1.8%. Conclusion: The method is simple, rapid, sensitive and can be used for direct determination of norfloxacin.