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目的了解同一地区不同年份CA16基因变异状况。方法在同一地区用同一方法分离CA16;随机抽取第一代细胞分离到的病毒株,测定VP1区基因序列,应用DNAstar和DNAman分子生物学软件对VP1基因进行分析比较。结果在2008年-2011年从104份标本中分离到47株CA16,病毒分离阳性率为45.2%;4年来,8株CA16核苷酸同源性为90.3%~100.0%,其中2010年分离的二株病毒核苷酸同源性为90.3%;8株CA16氨基酸同源性为97.9%~100.0%。结论由于在同一地区不同时间分离的CA16基因存在较大差异,因此选择CA16疫苗株时,应进行各种基因型及同一基因亚型中氨基酸变异较大毒株的交叉保护试验。
Objective To understand the variation of CA16 gene in different years in the same area. Methods The same method was used to isolate CA16 in the same area. The virus strains isolated from the first generation cells were randomly selected and the VP1 gene sequence was determined. The VP1 gene was analyzed and compared using DNAstar and DNAman molecular biology software. Results 47 strains of CA16 were isolated from 104 samples from 2008 to 2011, the positive rate of virus isolation was 45.2%. In the past 4 years, the nucleotide homology of 8 strains of CA16 was 90.3% -100.0% The nucleotide homology of the two viruses was 90.3%. The amino acid identities of the eight strains of CA16 were 97.9% -100.0%. Conclusion Because of the large discrepancy of CA16 gene in the same area at different times, the CA16 vaccine strain should be cross-protected when cross-protected with a large number of amino acid mutations in the same genotype and subgenotype.