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在模拟海拔６０００ｍ低氧实验条件下，观察了双后足冻伤对大鼠新鲜血浆和致冷血浆中冷凝蛋白（ＣＩＰ）含量的影响。冻前平原冻伤组（ＦＮ）和急性缺氧冻伤组（ＦＡＨ，冻前在模拟６０００ｍ高度停留４ｈ）大鼠新鲜血浆中ＣＩＰ含量分别为１０９．１±８．９μｇ／ｍｌ和１１０．１±６．２μｇ／ｍｌ（Ｐ＞０．０５），明显低于缺氧习服缺氧冻伤组（ＦＨＡＣ，模拟６０００ｍ高度每日停留４ｈ，共４周）的１３５．６±７．６μｇ／ｍｌ（Ｐ＜０．０５）。冻后４、２４、４８和７２ｈ，ＦＮ、ＦＡＨ和ＦＨＡＣ三组大鼠新鲜血浆和致冷血浆中ＣＩＰ含量均明显增高，除个别时间点外，ＦＡＨ和ＦＨＡＣ组新鲜血浆中ＣＩＰ含量均高于ＦＮ组。ＦＮ、ＦＡＨ和ＦＨＡＣ三组大鼠致冷血浆中ＣＩＰ含量均比相应时间点新鲜血浆中ＣＩＰ含量约高３倍以上。上述结果表明ＣＩＰ可能是一种应激蛋白，缺氧习服、冷损伤、冷冻过程中的低氧暴露及血浆冷藏均可使ＣＩＰ生成增多。
The effect of double hindfoot frostbite on the content of condensing protein (CIP) in fresh plasma and cryogenic plasma was observed under simulated hypoxia conditions at 6000m altitude. The fresh plasma CIP levels in the frozen-thawed frostbite group (FN) and the acute hypoxic-frostbite group (FAH, frozen at a simulated 6000 m altitude for 4 h before freezing) were 109.1 ± 8.9 μg / ml and 110.1 ± 6, respectively (P> 0.05), which was significantly lower than 135.6 ± 7.6μg / ml (P <0.05) in hypoxia acclimation hypoxic frostbite group (FHAC, simulating 6000m daily stay for 4h, <0.05). At 4, 24, 48 and 72 h after freezing, the levels of CIP in fresh plasma and cryogenic plasma of FN, FAH and FHAC groups were significantly increased, except for the individual time points, CIP content in fresh plasma of FAH and FHAC groups were higher than FN group. The levels of CIP in the plasma of the FN, FAH and FHAC groups were about three times higher than that of the fresh plasma at the corresponding time point. The above results suggest that CIP may be a stress protein, hypoxia acclimation, cold injury, hypoxia exposure in the process of freezing and plasma freezing can make CIP increased.