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目的探讨微小RNA(miR)-122和29在乙型肝炎病毒感染相关肝病中的表达水平及其临床意义。方法应用实时荧光定量PCR方法检测肝细胞癌(HCC)患者(20例)、肝炎肝硬化(LC)患者(20例)、慢性乙型肝炎(CHB)患者(15例)、乙型肝炎病毒携带者(ASC)(20例)以及正常对照(NC)(20例)血清中miRNA-122和29的表达水平,结合临床指标进行分析。结果与NC、LC和ASC组比较,HCC和CHB组患者血清中miR-122的表达水平明显升高;与NC比较,LC组患者血清中miR-29的表达水平明显下降,差异均具有统计学意义(P<0.01)。相关分析显示,CHB患者血清中miR-29表达与HBVDNA呈正相关;HCC患者血清中miR-29与AFP呈负相关。结论 miR-122的表达升高与CHB的肝损伤及HCC发生有关;miR-29的表达下降与肝纤维化有关,检测其变化有助于评价乙型肝炎病毒感染者肝脏的炎症损伤和纤维化。
Objective To investigate the expression of microRNA (miR) -122 and 29 in hepatopathy associated with hepatitis B virus infection and its clinical significance. Methods Twenty patients with hepatocellular carcinoma (HCC), 20 with liver cirrhosis (LC), 15 with chronic hepatitis B (CHB) and 15 with hepatitis B virus (ASC) (20 cases) and normal control (NC) (20 cases), and analyzed the clinical data. Results Compared with NC, LC and ASC groups, the serum levels of miR-122 in patients with HCC and CHB were significantly increased. Compared with NC, the levels of miR-29 in serum of patients with LC were significantly decreased, the differences were statistically significant Significance (P <0.01). Correlation analysis showed that there was a positive correlation between the expression of miR-29 in serum of patients with CHB and HBVDNA, and the negative correlation between serum miR-29 and AFP in patients with HCC. Conclusion The increased expression of miR-122 is associated with liver injury and HCC occurrence in CHB. The decreased expression of miR-29 is associated with hepatic fibrosis. To detect the changes of miR-122 is helpful to evaluate the inflammatory injury and fibrosis in the liver of patients with hepatitis B virus infection .