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目的探讨乙型肝炎病毒亚基因在肝细胞癌发生中的作用。方法以BamHI、BglⅡ酶切3.2kbHBVDNA,回收其HBs、HBx、HBc、PreS亚基因片段,以地高辛甙元标记成探针。以HBV探针Southern杂交分析其存在状态,以HBV亚基因探针Northern杂交检测亚基因转录。结果HBV阳性肝细胞癌中,整合型与混合型标本分别为63.6%和36.4%,纯整合型标本中,PreS、HBs、HBx、HBcmRNA阳性率分别为11.1%、44.4%、55.6%、11.1%。结论HBVDNA以整合于染色体上作为存在的主要方式,整合的HBVDNA有一定程度的转录,指导相应蛋白合成而影响肿瘤的发生。
Objective To investigate the role of hepatitis B virus subgenomics in the development of hepatocellular carcinoma. Methods 3.2kb HBV DNA was digested with BamHI and BglII, and the HBs, HBx, HBc and PreS subgenomic fragments were recovered and labeled with digoxin aglycone. The presence of HBV probes was analyzed by Southern blotting, and subgenomic transcription was detected by Northern hybridization of HBV subgenomic probes. Results In HBV positive hepatocellular carcinoma, 63.6% and 36.4% of the integrative and mixed specimens were detected. In the purely integrated specimens, the positive rates of PreS, HBs, HBx, and HBc mRNA were 11.1% and 44.4%, respectively. 4%, 55.6%, and 11.1%. Conclusion HBV DNA is integrated into the chromosome as the main method of existence. The integrated HBV DNA has a certain degree of transcription, which instructs the synthesis of the corresponding protein and affects the occurrence of the tumor.