Fabrication and characterization of YVO4:Eu3+ nanophosphors prepared by microwave(MW) irradiation assisted soft template synthesis were reported.The effects of synthesis conditions such as different powers of MW irradiation,pH values and concentration of reac-tion materials on properties of nanophosphor were also investigated to obtain the controllable size,morphology and high luminescence effi-ciency.Morphology,crystalline structure,and optical properties were characterized by field emission scanning electron microscopy(FE-SEM) ,X-ray diffraction(XRD) and fluorescence spectroscopy,respectively.The results showed that YVO4:Eu3+ nanophosphors were obtained by using diethyleneglycol(DEG) as soft template,with pH values in the range of 4 to 12,upon microwave irradiation from 300 to 900 W,at temperature of 80 oC.The high fluorescent YVO4:Eu3+ nanocrystals obtained with size from 15 nm down to 8 nm are more effec-tive to develop an ultrahigh sensitive fluorescent label for biomolecule,cell and tissue. Fabrication and characterization of YVO4: Eu3 + nanophosphors prepared by microwave (MW) irradiation assisted soft template synthesis were reported. These effects of synthesis conditions such as different powers of MW irradiation, pH values ​​and concentration of reac- tion materials on properties of nanophosphor were also Morphology, crystalline structure, and optical properties were characterized by field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) and fluorescence spectroscopy, respectively. The results showed that YVO4: Eu3 + nanophosphors were obtained by using diethyleneglycol (DEG) as soft template, with pH values ​​in the range of 4 to 12, upon microwave irradiation from 300 to 900 W, at temperature of 80 ° C. The high fluorescent YVO4 : Eu3 + nanocrystals obtained with size from 15 nm down to 8 nm are more effec-tive to develop an ultrahigh sensitive fluorescent label for biomolecule, cell and tissue.