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目的比较腺病毒重组CTLA4Ig/α4β7诱导的耐受性树突状细胞对卵清蛋白(OVA)致敏小鼠的预防和治疗效果。方法 BALA/c小鼠32只,分为4组。基础致敏24 h后回输耐受性树突状细胞为预防组;肠道激发4 h后回输耐受性树突状细胞为治疗组;回输生理盐水为阴性对照组和OVA过敏小鼠为阳性对照组。观察各组小鼠过敏症状缓解情况;HE染色观察空肠形态;甲苯胺兰染色观察小肠固有层肥大细胞聚集及脱颗粒现象;ELISA测定各组血清中OVA特异性IgE水平。结果与阳性对照组相比,治疗组小鼠OVA特异性IgE水平显著降低(0.25±0.05 vs 0.17±0.03)(P<0.05);体重增长明显(3.16 g±0.75 g vs 5.04 g±0.49 g)(P<0.05);腹泻症状减轻;HE染色可见治疗组小肠绒毛中上皮细胞局灶性坏死、脱落,固有层炎症细胞浸润现象明显改善;肥大细胞聚集和脱颗粒现象改善。而预防组较阳性对照组过敏症状、小肠形态学及OVA特异性IgE水平差异均无统计学意义。结论 Ad CTLA4Ig/Adα4β7修饰的致耐受性树突状细胞对卵清蛋白过敏小鼠具有治疗作用而无预防作用。
Objective To compare the preventive and therapeutic effects of dendritic cells induced by adenovirus recombinant CTLA4Ig / α4β7 on ovalbumin (OVA) sensitized mice. Methods 32 BALA / c mice were divided into 4 groups. After 24 h of sensitization, the dendritic cells were transfused into the prophylactic group, and the dendritic cells were transfused for 4 h after the priming into the treatment group. The saline-negative control group and the hypersensitivity to OVA Rats as positive control group. The allergic symptoms were observed in each group. The morphology of jejunum was observed by HE staining. Mast cell aggregation and degranulation were observed by toluidine blue staining. The serum levels of OVA-specific IgE were measured by ELISA. Results Compared with the positive control group, the OVA-specific IgE level in the treatment group was significantly decreased (0.25 ± 0.05 vs 0.17 ± 0.03) (P <0.05), and the body weight was significantly increased (3.16 g ± 0.75 g vs. 5.04 g ± 0.49 g) (P <0.05). The symptom of diarrhea was relieved. The HE staining showed focal necrosis and shedding of epithelial cells in the small intestine villi of the treated group, and the infiltration of inflammatory cells in the lamina propria obviously improved. The aggregation and degranulation of mast cells were improved. The prevention group than the positive control group allergy symptoms, small intestinal morphology and OVA-specific IgE levels were no significant difference. Conclusions Ad-CTLA4Ig / Adα4β7-induced tolerogenic dendritic cells have therapeutic and preventive effects on ovalbumin-allergic mice.